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Search over 300 published research articles on hypochlorous acid

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Results: 25 published articles

National Library of Medicine

0.01% Hypochlorous Acid as an Alternative Skin Antiseptic: An In Vitro Comparison

Microbe(s): National Library of Medicine


Abstract OBJECTIVE Compare the in vitro efficacy of hypochlorous acid 0.01% (HA), povidone iodine 5% (PI), chlorhexidine gluconate 4% (CHG), and isopropyl alcohol 70% (IPA) against common skin microorganisms. MATERIALS AND METHODS Time-kill studies were conducted against methicillin-susceptible Staphylococcus aureus (MSSA) and Staphylococcus epidermidis (MSSE), methicillin-resistant S. aureus...

NCBI - Oral Biofilm Bacteria

Effects of stabilized hypochlorous acid on oral biofilm bacteria

Microbe(s): NCBI - Oral Biofilm Bacteria


Low concentrations of HOCl (5 ppm), stabilized with 0.14% or 2% HAc, significantly reduced viability in multi-species biofilms representing supra- and sub-gingival oral communities, after 5 min, without causing erosion of HA surfaces. No equivalent antimicrobial effect was seen for CHX. Gram-positive and Gram-negative bacteria showed no significant differential suceptibility to stabilized...

NCBI - SARS-CoV-2-surrogate

Hypochlorous acid inactivates oral pathogens and a SARS-CoV-2-surrogate

Microbe(s): NCBI - SARS-CoV-2-surrogate


HOCl solution (45–60 ppm) is still effective against oral pathogens and SAR-CoV-2 surrogate viruses even in the presence of saliva and after passing through the dental unit water line. This study indicates that the HOCl solution can be used as therapeutic water or mouthwash and may ultimately reduce the risk of airborne infection in dental...

NCIB - Infectious Pathogens

The Effect of Hypochlorous Acid Disinfectant on the Reproduction of Details and Surface Hardness of Type III Dental Stone

Microbe(s): NCIB - Infectious Pathogens


A total of 100 samples of type III dental stone, 50 samples for each test, which include the reproduction of detail test and surface hardness test, were prepared and randomly divided into five groups, 10 samples for each test group, which include group A (control), group B (immersion in 200 ppm HOCl disinfectant for five minutes), group C (spraying with 200 ppm HOCl disinfectant for five...

BioMed Research International - Covid 19

Environmental Disinfection of a Dental Clinic during the Covid-19 Pandemic: A Narrative Insight

Microbe(s): BioMed Research International - Covid 19


Surface disinfection via aerosol (SDVA). The device produces dry fog through a turbine at high speed that atomized and sprays disinfectant. Usually, H2O2 and hypochlorous acid (HOCl) are used as a disinfectant (Figures 1 and 2). The disinfectant is atomized into ultrafine droplets, blown into the air, and, after 10-30 min, settles on all surfaces; these disinfectant droplets quickly begin to...

Brazilian Dental Journal

Viability and Effects on Bacterial Proteins on Dental Oral Rinses with Hypochlorous Acid as Active Ingredient

Microbe(s): Brazilian Dental Journal


Hypochlorous acid rinses are proposed for plaque control and as a wound healing agent for its use in oral health, due to its low toxicity, high antimicrobial efficacy, anti-inflammatory effect, induction to cell proliferation and its background as a topical solution in the antisepsis of wounds in clinical medicine 16. HOCl has also the ability to oxidize the amino acid taurine and induce the...

Environmental Health Science and Engineering 13.1 (2015): 6

Suitability of electrolyzed oxidizing water for the disinfection of hard surfaces and equipment in radiology

Microbe(s): Environmental Health Science and Engineering 13.1 (2015): 6


Hospitals are faced with increasingly resistant strains of micro-organisms. When it comes to disinfection, individual parts of electronic equipment of angiology diagnostics such as patient couches of computer tomography (CT) and magnetic resonance imaging (MRI) scanners prove to be very hard to disinfect. Disinfectants of choice are therefore expected to possess properties such as rapid,...

Journal of Trauma and Acute Care Surgery 49.3 (2000): 511-514

Effectiveness of electrolyzed oxidized water irrigation in a burn-wound infection model

Microbe(s): Journal of Trauma and Acute Care Surgery 49.3 (2000): 511-514


OBJECTIVE: The purpose of the study was to determine whether electrolyzed oxidized water (EOW) functions as a bactericide in burn injury with Pseudomonas aeruginosa infection in a rat burn-wound model. METHODS:Anesthetized Sprague-Dawley rats (n = 31) were subjected to third-degree burns to 30% of total body surface area. Two days after injury, all rats were infected with P. aeruginosa using 1 mL...

Artificial organs 24.12 (2000): 984-987

Effect of electrolyzed water on wound healing

Microbe(s): Artificial organs 24.12 (2000): 984-987


Electrolyzed water accelerated the healing of full-thickness cutaneous wounds in rats, but only anode chamber water (acid pH or neutralized) was effective. Hypochlorous acid (HOCl), also produced by electrolysis, was ineffective, suggesting that these types of electrolyzed water enhance wound healing by a mechanism unrelated to the well-known antibacterial action of HOCl. One possibility is that...

Digestive Endoscopy 15.1 (2003): 19-24

Endoscope contamination from HBV- and HCV-positive patients and evaluation of a cleaning/disinfecting method using strongly acidic electrolyzed water

Microbe(s): Digestive Endoscopy 15.1 (2003): 19-24


Background: It is well known that strongly acidic electrolyzed water (SAEW) has a potent bactericidal effect. We examined residual viruses on endoscopes that were used in hepatitis B virus (HBV)-positive and hepatitis C virus (HCV)-positive patients and evaluated the effectiveness of SAEW in cleaning/disinfecting the endoscopes. Methods: A random sample of endoscopes used in 109 endoscopies on...

Journal of Gastroenterology and Hepatology 19.8 (2004): 897-903

Efficacy of electrolyzed acid water in reprocessing patient used flexible upper endoscopes: Comparison with 2% alkaline glutaraldehyde

Microbe(s): Journal of Gastroenterology and Hepatology 19.8 (2004): 897-903


Background and Aim: Two percent glutaraldehyde, the most widely used liquid chemical germicide (LCG), may be hazardous to patients and medical personnel. Alternatives to glutaraldehyde, such as electrolyzed acid water (EAW), are being developed, but data from well-controlled studies with patient-used endoscopes are rare. The purpose of the present paper was to evaluate the high-level...

Chinese Journal of Nosocomiology 4 (2009): 031

Effect of Disinfection by Acidic Electrolyzed Oxidizing Water on Medical Instrument [J]

Microbe(s): Chinese Journal of Nosocomiology 4 (2009): 031


OBJECTIVE To realize the disinfectant effect of medical instrument by acidic electrolyzed oxidizing water(EOW).METHODS Two groups of subjects participated in this study,and chlorinated disinfectant is used in the control group. Adopting stability test to measure effective chlorine and pH,and to compute the bacterium elimination rate after disinfection. Contrast the result with the experimental...

Surgery Today 39.6 (2009): 514-517

Effect of electrolyzed strong acid water on peritoneal irrigation of experimental perforated peritonitis

Microbe(s): Surgery Today 39.6 (2009): 514-517


PURPOSE: Electrolyzed strong acid water (ESAW) is generated by the electrolysis of a weak sodium chloride solution. Although ESAW is known to have a strong bactericidal activity and to be harmless to the living body, its effectiveness and safety in the treatment of perforated peritonitis has not been well established. METHODS: Male Wistar rats were used for the study. Three hours after cecal...

European journal of clinical microbiology & infectious diseases 31.5 (2012): 641-653

Electrochemically activated solutions: evidence for antimicrobial efficacy and applications in healthcare environments

Microbe(s): European journal of clinical microbiology & infectious diseases 31.5 (2012): 641-653


Due to the limitations associated with the of existing biocidal agents, there is a need to explore new methods of disinfection to help maintain effective bioburden control, especially within the healthcare environment. The transformation of low mineral salt solutions into an activated metastable state, by electrochemical unipolar action, produces a solution containing a variety of oxidants,...

Canadian Journal of Microbiology 58.4 (2012): 448-454

Efficacy of acidic and basic electrolyzed water in eradicating Staphylococcus aureus biofilm

Microbe(s): Canadian Journal of Microbiology 58.4 (2012): 448-454


Staphylococcus aureus is a major pathogen. It can form biofilm on the surfaces of medical devices and food equipment, which makes it more difficult to eradicate. To develop a novel method to eradicate S. aureus biofilm, the effects of electrolyzed water on removing and killing S. aureus biofilm were investigated in this study. By using a biofilm biomass assay with safranin staining and...

Int J Artif Organs 36.11 (2013): 781-92

Sterilization using electrolyzed water highly retains the biological properties in tissue-engineered porcine liver scaffold

Microbe(s): Int J Artif Organs 36.11 (2013): 781-92


PURPOSE: The aims of this study were to investigate the effects of sterilization with peracetic acid (PAA) and ethanol on the biological activity of porcine liver scaffolds and to develop a new technique for sterilization using slightly acidic electrolyzed water (SAEW). METHODS: Decellularization of liver slices was performed using 0.1% sodium-dodecyl-sulfate, then evaluated by histological and...

Infection Control & Hospital Epidemiology 35.12 (2014): 1505-1510

Evaluating use of neutral electrolyzed water for cleaning near-patient surfaces

Microbe(s): Infection Control & Hospital Epidemiology 35.12 (2014): 1505-1510


This study aimed to monitor the microbiological effect of cleaning near-patient sites over a 48-hour period with a novel disinfectant, electrolyzed water. One ward dedicated to acute care of the elderly population in a district general hospital in Scotland. Lockers, left and right cotsides, and overbed tables in 30 bed spaces were screened for aerobic colony count (ACC), methicillin-susceptible...

Annals of Clinical Microbiology and Antimicrobials 13.1 (2014): 29

In vitro antimicrobial activity of Medilox super-oxidized water

Microbe(s): Annals of Clinical Microbiology and Antimicrobials 13.1 (2014): 29


Super-oxidized water is one of the broad spectrum disinfectants, which was introduced recently. There are many researches to find reliable chemicals which are effective, inexpensive, easy to obtain and use, and effective for disinfection of microorganisms leading hospital infections. Antimicrobial activity of super-oxidized water is promising. The aim of this study was to investigate the in-vitro...

Wounds 27.10 (2015): 265-273

A new acid-oxidizing solution: assessment of its role on methicillin-resistant Staphylococcus aureus (MRSA) biofilm morphological changes

Microbe(s): Wounds 27.10 (2015): 265-273


OBJECTIVE: Biofilms represent a key challenge in the treatment of chronic wounds, as they are among the main reasons for delays in chronic wound healing. This in vitro study was aimed at evaluating the activity of a new acid-oxidizing solution (AOS) on biofilm formation. Acid-oxidizing solution contains free chlorine species with stabilized hypochlorous acid in high concentration (> 95) and is...

International journal of clinical and experimental medicine 8.7 (2015): 11463

An nvestigation into the in-vitro effectiveness of electrolyzed water against various microorganisms

Microbe(s): International journal of clinical and experimental medicine 8.7 (2015): 11463


The aim of this study was to investigate the in-vitro antimicrobial activity of usage and normal concentrations of electrolyzed water in hospital. In our study, the effects of different concentrations of electrolyzed water on two gram positive, four gram negative standard strains and clinical isolates of four gram negative, two gram positive, one spore-forming bacillus and Myroides spp strains...

Surgery today 45.7 (2015): 876-879

Efficacy and safety of strong acid electrolyzed water for peritoneal lavage to prevent surgical site infection in patients with perforated appendicitis

Microbe(s): Surgery today 45.7 (2015): 876-879


Our previous experimental study of perforated peritonitis in rats proved that peritoneal lavage with strong acid electrolyzed water (SAEW) has no adverse effects, reduces the bacteria count in the ascitic fluid more effectively than saline, and increases the survival rate significantly. Thus, we conducted a randomized controlled study, applying SAEW in the treatment of perforated appendicitis in...

Eplasty 16 (2016)

The Immediate and Delayed Post-Debridement Effects on Tissue Bacterial Wound Counts of Hypochlorous Acid Versus Saline Irrigation in Chronic Wounds

Microbe(s): Eplasty 16 (2016)


Introduction: Wound debridement is considered essential in chronic wound management. Hypochlorous acid has been shown to be an effective agent in reducing wound bacterial counts in open wounds. Ultrasound-enabled wound debridement is an effective and efficient method of debridement. This study compared ultrasound irrigation with hypochlorous acid versus saline irrigation for wound debridement on...

The Laryngoscope 126.4 (2016): 791-795

Improved outcomes after low-concentration hypochlorous acid nasal irrigation in pediatric chronic sinusitis

Microbe(s): The Laryngoscope 126.4 (2016): 791-795


Objectives/Hypothesis We aimed to evaluate the effectiveness of low-concentration hypochlorous acid (HOCl) nasal irrigation compared to isotonic normal saline for pediatric chronic rhinosinusitis. Study Design This was a randomized, prospective, active-controlled study.MethodsThis study investigated the effectiveness of 4 weeks of low-concentration hypochlorous irrigation by analyzing five...

International Journal of Molecular Sciences 17.7 (2016): 1161

Effectiveness of hypochlorous acid to reduce the biofilms on titanium alloy surfaces in vitro

Microbe(s): International Journal of Molecular Sciences 17.7 (2016): 1161


Chemotherapeutic agents have been used as an adjunct to mechanical debridement for peri-implantitis treatment. The present in vitro study evaluated and compared the effectiveness of hypochlorous acid (HOCl), sodium hypochlorite (NaOCl), and chlorhexidine (CHX) at eliminating Gram-negative (E. coli and P. gingivalis) and Gram-positive (E. faecalis and S. sanguinis) bacteria. The effect of...

Clinical Ophthalmology (Auckland, NZ) 11 (2017): 707

Reduction in bacterial using hypochlorous acid hygiene solution on ocular skin

Microbe(s): Clinical Ophthalmology (Auckland, NZ) 11 (2017): 707


Purpose To examine the magnitude of bacterial reduction on the surface of the periocular skin 20 minutes after application of a saline hygiene solution containing 0.01 pure hypochlorous acid (HOCl). Methods Microbiological specimens were collected immediately prior to applying the hygiene solution and again 20 minutes later. Total microbial colonies were counted and each unique colony morphology...